Bcl-2 anti-sense oligonucleotide sensitizes Fas-mediated apoptosis of gastric cancer cells
Cao, J.; Qiao, Y.; Min, J.
Zhonghua Zhong Liu Za Zhi 22(6): 466-468
2000
ISSN/ISBN: 0253-3766 PMID: 11235565 Document Number: 523660
To study the effect of bcl-2 anti-sense oligonucleotide on the sensitivity of gastric cancer cells to Fas-mediated apoptosis. Gastric cancer cell line MKN45 was transfected with bcl-2 anti-sense oligonucleotide, and expression of bcl-2 was examined by Western blotting. Agonistic Fas antibody was used to induce apoptosis detected by TUNEL staining and flow cytometry. Bcl-2 expression in MKN45 cells transfected with bcl-2 anti-sense oligonucleotide was markedly inhibited. When cultured with antibody apoptosis index of the anti-sense oligonucleotide-treated MKN45 cells was 55.6% +/- 4.7% (n = 5), which was significantly higher than that of the control (8.4% +/- 2.1%, n = 5). Expression of bcl-2 in gastric cancer cells may antagonize Fas-mediated apoptosis.