Nested RT-PCR in detection of blood AFPmRNA in animal model of human hepatocellular carcinoma
Liu, Y.; Wu, M.-C.; Qian, G.-X.; Zhang, B.-H.
Hepatobiliary and Pancreatic Diseases International Hbpd Int 2(3): 414-417
2003
ISSN/ISBN: 1499-3872 PMID: 14599950 Document Number: 558739
To detect blood AFPmRNA in the nude mice bearing with human hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR), and assess its significance in HCC distant metastasis. We detected 20 blood samples from the nude mice bearing with human HCC by nested RT-PCR to find out AFPmRNA. AFPmRNA was detected in 6 blood samples from the nude mice bearing with human HCC (30.0%), in which 4 mice developed distant metastasis. AFPmRNA may be used as an efficient and sensitive marker to detect blood spread of HCC cells. It can predict the occurrence of HCC distant metastasis.