A modified rat model for hepatocellular carcinoma
Yang, F.-C.; Zheng, S.-S.; Jiang, T.-A.
Hepatobiliary and Pancreatic Diseases International Hbpd Int 3(4): 585-587
2004
ISSN/ISBN: 1499-3872 PMID: 15567750 Document Number: 582875
Rat hepatocellular carcinoma (HCC) model which has a high analogy to clinical liver cancer is of great value in understanding the pathogenesis and evolution of liver cancer, in searching effective anti-cancer treatments (drug, hepatectomy and liver transplantation), and designing cancer prevention strategies. In this study we established a modified rat model of hepatocellular carcinoma to enhance rats' physique and surgical endurance. Wistar rats were fed with diethylnitrosamine (DENA) by three methods for evaluation of general conditions for 130 days: Doppler ultrasonographic measurement, laparotomy and histopathological examination. No rat died in control group (group A) and modified DENA-induction-HCC group(group C), but 6 deaths in classical DENA-induction-HCC group (group B) (survival rate 80%). All survived rats in groups B and C developed diffusive hepatocellular carcinoma and liver cirrhosis. General appearance of rats in the group C was better than that in the group B. With good general conditions for surgery, the modified rat model for hepatocellular carcinoma has a high carcinogenic rate and a high survival rate.