Method for determining nitrogen-containing cationic surface-active agents using butyrylcholinesterase
Zhukovskiĭ, I.G.; Kuznetsova, L.P.; Sochilina, E.E.
Ukrains'kyi Biokhimichnyi Zhurnal 74(2): 140-143
2002
ISSN/ISBN: 0372-3909 PMID: 12152307 Document Number: 551109
The biochemical method for determination of cetyltrimethyl ammonium or cetylpyridinium, both being nitrogenated cationic surfactants, has been devised by using horse blood serum butyrylcholinesterase as analytical reagent. The method streams from the fact that surfactants tested are inhibitors of butyrylcholinesterase hydrolysis of butyrylcholin, a cationic substrate, but in this case they activate enzymatic hydrolysis of 1-naphthylacetate, a neutral substrate. Presence two opposite effects enlarges reliability to identifications. Use the sensitive fluorimetric method to registrations of activation of hydrolysis a substrate 1-naphtylacetate vastly to reduce the threshold of determination of surfactants above.