Cytometric method of determining the parameters of the interaction of synthetic immunostimulants with the lymphocytic surface
Shcherbukhin, V.V.; Zasypkin, D.V.; Ataullakhanov, F.I.; Skuĭbin, B.G.
Tsitologiia 30(10): 1218-1225
1988
ISSN/ISBN: 0041-3771 PMID: 3245090 Document Number: 313924
The cytofluorimetric method was suggested for the determination of parameters of polymer adsorption on the cellular surface. Multiple equilibrium in the homogeneous cellular population with N independent binding sites and in the heterogeneous population of cells with the Poisson distribution of adsorption sites was analysed. A possibility was shown of cytofluorimetric drawing of the adsorption isotherm using mean fluorescence intensity of cells in the equilibrium suspension. The equilibrium association constant and the mean number of binding sites were estimated for adsorption of poly-L-lysin on the surface of human myelogenous leukemic cells (cellular line K-252).