Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage
Zhang, H.-x.; Li, X.-q.; Tang, T.
Hunan Yi Ke da Xue Xue Bao 27(1): 38-40
2002
ISSN/ISBN: 1000-5625 PMID: 12575231 Document Number: 549212
To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao-yi-an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats. ICH models anesthesized were induced by stereotaxical injection of 2 microliters normal saline containing 0.4 U bacterial collagenase(type VII) into the right globas pallidus(GP), and the protein expression of bcl-2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed. Bcl-2 positive cells were found in the cerebral cortex and basal ganglia area after ICH. Immunoreactivity was localized in the cytoplasm as a fine granular precipitate. The levels of bcl-2 expression in the model group varied at different time-points, and reached the peak at 2 d. In the NYA group, the protein expression of bcl-2 was increased significantly both in the cortex and in the basal ganglia compared with those in the model group. NYA can significantly upregulate the protein expression of bcl-2.