Effect of exogenous hyaluronan on wound healing
Liu, D.-l.; Li, X.-j.; Zhang, Y.; Li, Y.-x.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 16(4): 256-258
2002
ISSN/ISBN: 1002-1892 PMID: 12181791 Document Number: 547681
To investigate the mechanism of impeded wound healing by exogenous hyaluronan (HA). Wound healing models were established on 18 adult rabbit ears, which were randomly divided into 3 groups, the 2% HA treated-group (group A), the 1% HA treated-group(group B), and the PBS control group (group C). The process of wound healing was observed morphologically and histologically. The expression of alpha-smooth muscle actin in fibroblast was measured by immunohistochemical method. 1. The mean values of wound healing time of groups A, B and C were (11.7 +/- 0.6), (11.3 +/- 0.6), and (10.8 +/- 1.0) days respectively. Wound contraction was greater in group C than in group A and group B. 2. Compared with PBS controls, the collagen fibril was slender and arrayed regularly in HA treated wound. 3. The expression of alpha-smooth muscle actin was greater in group C than in groups A and B. It is one of reasons of impeded wound healing that exogenous HA inhibits the expression of alpha-smooth muscle protein and wound contraction. There exists dose-dependent effect.