Platelet derived wound healing factors (PDWHF) accelerate and augment wound healing angiogenesis in the rat
Phillips, G.D.; Stone, A.M.; Whitehead, R.A.; Knighton, D.R.
In Vivo 8(2): 167-171
1994
ISSN/ISBN: 0258-851X PMID: 7522605 Document Number: 427982
The object of this study was to determine the effect of PDWHF on wound healing angiogenesis. Sponges saturated with PDWHF were implanted in rats. Six hours to 14 days post-implantation, vascular corrosion casts were prepared and examined by SEM. Leukocyte margination and angiogenesis was accelerated by 24-48 hours. Vessel number was also greater than in the controls. Another series of PDWHF treated sponges were implanted. Fourteen days later the vasculature was perfused with Evans blue. Sponges were homogenized, and absorbance of the supernatants was determined. Absorbances of PDWHF supernatants was 2 times greater than controls. PDWHF enhanced wound healing angiogenesis in the rat.