An improved method for rapid ABO genotyping
Vanĕk, D.; Pospísek, M.
Sbornik Lekarsky 103(1): 29-33
2002
ISSN/ISBN: 0036-5327 PMID: 12448935 Document Number: 547129
A method for ABO genotyping originally designed by Lee and Chang [3] and further developed by Akane et al. [7] has been even more simplified and improved. We obtained a rapid, robust, sensitive and low cost method for detection of sequence polymorphism of ABO glycosyltransferase gene by changing the MaeII restriction enzyme for its isoschizomer TaiI and by optimization of the condition during digestion and electrophoretic separation.
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