A method of genotyping clinical isolates of Ureaplasma urealyticum biovar Parvo
Ekimov, A.N.; Zagrebina, O.S.; Kisina, V.I.; Govorun, V.M.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1: 28-32
2001
ISSN/ISBN: 0208-0613 PMID: 11236738 Document Number: 536349
A new method for typing clinical isolates of U. urealyticum (Parvo biovar) is based on SSCP analysis of amplicons of mba gene 5' region and upstream region. The mba gene is coding for MB gene of U. urealyticum. This method allows genotyping of U. urealyticum isolates using vaginal and cervical swabs without culturing. Sixty-two clinical specimens from patients with a history of chronic cystitis, chronic pyelonephritis, chronic salpingo-oophoritis, erosion of the cervix uteri, and spontaneous abortions were tested for U. urealyticum. The bacterium was detected in 64% (40 specimens), 83% (33) of which belonged to Parvo biovar. Parvo biovar isolates were analyzed and genotyped as follows: first genotype 52%, second genotype 33%, and third genotype 16%. Further sequencing of the first and second genotype amplicons showed that the first genotype belonged to serotype 3 and second genotype to serotype 6.