Vitamin E potentiates the antiplatelet activity of aspirin in collagen-stimulated platelets
Celestini, A.; Pulcinelli, F.M.; Pignatelli, P.; Lenti, L.; Frati, G.; Gazzaniga, P.Paolo.; Violi, F.
Haematologica 87(4): 420-426
2002
ISSN/ISBN: 0390-6078 PMID: 11940487 Document Number: 544597
Aspirin-pretreated platelets were incubated with vitamin E, at concentrations usually found in peripheral circulation after oral supplementation, to determine whether vitamin E can enhance the inhibitory effect of aspirin in collagen-stimulated platelets, and whether vitamin E reduces the platelet-collagen interaction in aspirin-pretreated platelets. Vitamin E, at a concentration of 50 micro M and 100 micro M, inhibited platelet adhesion to collagen (80% and almost total inhibition, respectively). In the presence of vitamin E, a higher collagen concentration was needed to obtain 50% of maximal platelet activation (P<0.0001, vs. untreated platelets). Platelet secretion induced by collagen was inhibited by vitamin E. Vitamin E reduced the collagen concentration able to induce 50% of maximum ATP release in aspirin-treated platelets (P<0.005, vs. untreated platelets). Collagen-induced Ca2+ mobilization was also inhibited by vitamin E in aspirin-treated platelets. In the presence of high doses of vitamin E, a greater collagen concentration was needed to obtain 50% of maximal calcium mobilization.