High-throughput array production using precision glass syringes
Shieh, J.; To, C.; Carramao, J.; Nishimura, N.; Maruta, Y.; Hashimoto, Y.; Wright, D.; Wu, H.C.; Azarani, A.
Biotechniques 32(6): 1360-2 1364-5
2002
ISSN/ISBN: 0736-6205 PMID: 12074167 Document Number: 544011
The advantages of using 1, 96, or 384 precision glass syringes in automated high-throughput microdispensers in creating highly uniform and reproducible DNA, protein, and organic compound array filters and slides are described. Using the Hydra(R) Microdispenser and TangoTM Liquid Handling system, 0.1-5 ng (in 50-300 nL) PCR-amplified, human cancer-related genes and housekeeping genes were spotted onto nylon membranes and coated slides. Protein solutions of 50 mug/mL to 1 mg/mL were spotted onto coated slides or onto MaxiSorpTM 96-well plates. Up to 6144 spots/membrane and up to 1000 spots/slide were printed. The size of the spots created by glass syringes was uniform and reproducible (precision variation of less than 5%) from spot to spot and membrane to membrane. Using a Tango 384 system, a total of ten 6144-spot filters can be produced in approximately 25 min, translating into a spotting speed of 2.5 min/membrane.