High throughput screening for intercellular adhesion molecule-1 inhibitor
Ren, D-cheng.; Du, G-hua.; Zhang, J-tian.
Yao Xue Xue Bao 38(6): 405-408
2003
ISSN/ISBN: 0513-4870 PMID: 14513796 Document Number: 555901
Aim: To develop a high throughput screening assay to identify inhibitors of intercellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVEC). Methods: ICAM-1 expression in lipopolysaccharide-stimulated endothelial cells was measured by ELISA. The cytotoxicity of the compounds was measured by MTT. Results: Lipopolysaccharide (LPS) increased ICAM-1 expression in HUVEC in a concentration- and time-dependent manner. Two thousand compounds were screened and the hit rate was 1.5%. Among these 30 compounds, 24 were cytotoxic. Conclusion: The ELISA method was inexpensive, reproducible and suitable for high throughput primary cell assay. This assay was feasible to identify inhibitors of ICAM-1 and simultaneously discriminate the activity from the cytotoxic effects.