High-throughput techniques for compound characterization and purification
Kyranos, J.N.; Cai, H.; Zhang, B.; Goetzinger, W.K.
Current Opinion in Drug Discovery and Development 4(6): 719-728
2001
ISSN/ISBN: 1367-6733 PMID: 11899611 Document Number: 540474
A new paradigm in drug discovery is the synthesis of structurally diverse collections of compounds, so-called libraries, followed by high-throughput biological screening. High-throughput characterization and purification techniques are required to provide high-quality compounds and reliable biological data, which has led to the development of faster methods, system automation and parallel approaches. This review summarizes recent advances in support of analytical characterization and preparative purification technologies. Notably, mass spectrometry (MS) and supercritical fluid chromatography (SFC) are among the areas where new developments have had a major impact on defining these high-throughput applications.