Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm
Zhan, Y.; Zhao, Y.-N.; Li, Z.-L.; Li, L.; Song, Q.
Zhonghua Nan Ke Xue 8(2): 92-94
2002
ISSN/ISBN: 1009-3591 PMID: 12479017 Document Number: 543275
To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome. Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe. 1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01). KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.