Effect of the lipophilic spin-labeled inhibitor of cytochrome P-450 on the activity of the microsomal system
Subkhankulova, T.N.; Liakhovich, V.V.; Vaĭner, L.M.
Biokhimiia 52(7): 1186-1191
1987
ISSN/ISBN: 0320-9725 PMID: 3663758 Document Number: 292150
It was shown that the lipophilic nitroxyl radical.sbd.2-hexyl-2,3,5,5-tetramethyl-4-(3-iodo-2-oxopropyliden)-imidazolidine-1-oxyl, an affinity modified of rat liver microsomal cytochrome P-450, interacts with various forms of cytochrome P-450 as substrate type I, and it inhibits the oxidation of substrates specific for these forms. During its intravenous injection with egg phosphatidylcholine liposomes the radical is partly bound to liver microsomes which is accompanied by a decrease of the oxygenase activity of microsomal preparations (by 30-50%) as well as by prolongation of the soporific effect of hexabarbital (2-3-fold).