Inducible expression of non-structural protein 3 of hepatitis C virus in E. coli
Cheng, J.; Zhong, Y.; Liu, Y.; Dong, J.; Yang, J.; Yang, S.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 16(1): 85-87
2002
ISSN/ISBN: 1003-9279 PMID: 11986756 Document Number: 542582
To express recombinant non-structural protein 3 of hepatitis C virus (HCV) in E. coli. The non-structural 3 (NS3) region DNA fragment of HCV was amplified by polymerase chain reaction (PCR) and inserted into inducible proeukaryotic expressive vector pET 30C(+)at Bam H1/EcoR1 sites. The competent BL21 (DE3) E.coli was transformed, and then cultured and induced with IPTG. The expressed HCV NS3 protein was confirmed with ELISA and dot blot hybridization using HCV NS3-specific single chain Fv (ScFv) antibody. 1 893 bp DNA fragment of HCV NS3 coding region was amplified by PCR technique. HCV NS3 expressive vector pET-NS3 was constructed. After transformation with pET-NS3 and induction with IPTG, recombinant HCV NS3 protein was expressed and confirmed by specific ELISA and dot blot hybridization. The recombinant HCV NS3 can be expressed in E. coli.