Cloning, sequencing and expression of hepatitis e virus structural gene in E. coli and application of the recombinant products for diagnosis
Rong, G.; Sun, J.; Zhou, J.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 12(2): 169-172
1998
ISSN/ISBN: 1003-9279 PMID: 12515200 Document Number: 489979
The prokaryotic expression vector PEGX-3X was used to express hepatitis E virus (HEV) open reading frame 2(ORF2 402-660). The recombinant protein was soluble in PBS buffer and was purified by the glutathione Sepharose 4B affinity column. When the purified recombinant protein was applied to detect HEV antibodies in sera of clinical patients, the results were quite consistent with the HEV diagnostic kit from Diagnostic Biotechnology Ltd(DBL), Singapore. The recombinant protein was more reactive with HEV antibodies than DBL reagent.