Determination of lamotrigine in the serum and study on pharmacokinetics in rabbits by RP-HPLC
Hu, Y.S.; Wu, P.; Tang, Q.H.
Se Pu 19(4): 323-325
2001
ISSN/ISBN: 1000-8713 PMID: 12545490 Document Number: 540419
In the treatment of epileptic patients a method was developed to monitor the lamotrigine concentration by HPLC using a YWG-ODS column and a detector at 305 nm. The linear range was from 0.25 mg/L to 50 mg/L with a mean correlation coefficient of 0.9998. The average recovery was 93.78% and the standard deviation was 2.66%. Within and between-day precisions were 2.4% (n = 9) and 5.3% (n = 5) in RSD respectively. The pharmacokinetics was studied after a single dose of 25 mg/kg for 3 rabbits. Lamotrigine concentrations in serum were assayed and the parameters were computed with 3P87 program. The concentration-time curve of lamotrigine in rabbits fitted to a two compartment model, in which Cmax was 9.34 micrograms/mL, t1/2(Ke) was 8.78 h, and CL/f(s) was 0.69 L/h. The method is rapid, accurate and can be used as routine test in the use of lamotrigine.