Application of colloidal gold to ultrastructural studies
Seidel, J.; Zabel, M.
Folia Histochemica et Cytobiologica 39(2): 107-108
2001
ISSN/ISBN: 0239-8508 PMID: 11374780 Document Number: 539607
Progress in recognition of structure and function in a variety of cells has always been linked to development of new research techniques. Beyond doubt, immunocytochemical techniques belong to such new methods. However, the techniques had not been widely applied to electron microscopy until colloidal gold was introduced as a label. This paper presents the pre-embedding and the post-embedding techniques and the applied markers, which are used in immunocytochemical techniques at the ultrastructural level. The potential of colloidal gold techniques is discussed. Particular attention is paid to methods of reaction amplification. Application of gold is illustrated with our own results.