Chronically HIV-1 infected human monocyte culture U937 as a model for assessing the efficacy of anti-HIV agents
Pokrovskiĭ, A.G.; Pliasunova, O.A.; Gashnikova, N.M.; Mamaeva, O.A.; Fediuk, N.V.
Voprosy Virusologii 46(6): 38-42
2001
ISSN/ISBN: 0507-4088 PMID: 11785387 Document Number: 538249
Cell culture U937 chronically infected with HIV-1 is suggested as a model for adequate evaluation of antiviral activity of HIV inhibitors. Azidothimidine (AZT) notable decreased HIV-1 reproduction in chronically infected U937 cells to passages 15-18. Glycirrhizic acid (GA) effectively inhibited the virus production during the first four passages, while in subsequent passages (up to passage 20) decreased the virus production by only 60% in comparison with the control. If a combination of AZT and GA (1:1000) was used, p24 was not detected in the culture fluid by passage 20. Culturing of U937 cells with AZT led to a 10-fold decrease in the amount of DNA 2-LTR in comparison with the total content of proviral DNA, the content of HIV-1 DNA 1-LTR remaining virtually unchanged. Culturing of U937 with a combination of AZT and GA resulted in a notable decrease in the content of proviral DNA 2-LTR after passage 3, while after passage 9 this form of HIV-1 DNA was not detected at all.