Feasibility study on the detection of ferritin using surface plasmon resonance
Werawatgoompa, S.; Sriyudthsak, M.
Journal of the Medical Association of Thailand 84(Suppl): S155-S162
2001
ISSN/ISBN: 0125-2208 PMID: 11529329 Document Number: 537364
Determination of ferritin concentration was performed using surface plasmon resonance technique. An anti-human ferritin antiserum from rabbits was immobilized on the protein-A coated sensor chip. The resonance change due to the binding of ferritin to the antibody was measured. It was found that the antigen-antibody binding of the ferritin could be monitored in real time. The detection of ferritin could be obtained in the range of 25apprx800 ng/ml. This dynamic range could be applied in various medical applications. Moreover, it was observed that the sensor chips could be regenerated. This offers an opportunity for multiple uses, which will reduce the unit operating cost.