Studies on the manufacture and immunogenicity of purified rabies vaccines on humans Vero cell
Yu, W.; Liu, Z.; Qian, H.; Lu, H.; Wang, H.; Li, F.; Dou, Z.
Zhonghua Liu Xing Bing Xue Za Zhi 22(1): 17-19
2001
ISSN/ISBN: 0254-6450 PMID: 11860837 Document Number: 536649
Using Vero cell as basic cultural material to improve the quality of rabies vaccines and to produce rabies vaccines for humans. CTN-1V10 strain were used for production. Vero cell of 150th generation were used for cultivation. Rotating cultivatal method with rotating bottle was used. Fluids with virus were collected at different time. Puritied rabies vaccine was produced on Vero cell after clarification, condensation, purification and extermination. A batch of vaccines made by this techniques were used for immunological observation. Sixty-three people were injected with this rabies vaccine according to the procedure of time of exposure. Thirty of them were injected with vaccines made in France (Verorab) while the others were injected with vaccines to be tested. Side effect and neutralizing antibody were recorded. The quality of this newly developed rabies vaccines has met the quality set by WHO. After all dosages of injection, the rates of positive antibody were both 100% in two groups. The neutralizing antibody among testing group was 11.94 IU/ml comparing with control as 11.69 IU/ml. Purified rabies vaccines on Vero cell for humans had reasonable manufacture technique and less little effect with good technological imnunogenicity.