Fluconazole susceptibility of Italian Candida dubliniensis clinical isolates determined by reference and simplified tests
Giammanco, G.M.; Pecorella, S.; Distefano, S.; Pecoraro, V.; Milici, M.E.; Pizzo, G.
New Microbiologica 24(4): 397-404
2001
ISSN/ISBN: 1121-7138 PMID: 11718378 Document Number: 532402
Candida dubliniensis is an opportunistic pathogen mainly associated with oral candidiasis in human immunodeficiency virus (HIV)-infected individuals. We recently recovered the first Italian clinical isolates of C. dubliniensis from the oral cavities of seven HIV-seropositive subjects. The in vitro susceptibility to fluconazole (FLCZ) of these isolates was determined according to the National Committee for Clinical Laboratory Standards (NCCLS) M27-A broth microdilution method for yeasts. All seven isolates of C. dubliniensis were susceptible to FLCZ (MICs<=0.5 micro g/ml). Results of this reference method were compared to those obtained with simplified tests, more adapted to routine evaluation in hospital laboratories. Fungitest and Sensititre YeastOne colorimetric microplate-based methods have been evaluated. The agar disk diffusion method has also been tested on two different media: RPMI 1640-2% glucose and High Resolution-2% glucose-0.5 micro g/ml methylene blue. All of the simplified methods tested were able to correctly identify FLCZ-susceptibility of this group of Italian C. dubliniensis isolates.