Effect of partial and sham hepatectomy on the growth of a hepatocellular carcinoma
Barbeito, C.G.; Garcia, M.N.; Flamini, M.A.; Andrini, L.B.; Badrán, A.F.
Journal of Experimental and Clinical Cancer Research Cr 20(1): 153-158
2001
ISSN/ISBN: 0392-9078 PMID: 11370822 Document Number: 530566
We examined the effect of partial hepatectomy on the proliferation of hepatoma ES12a grafted into C3H/S mice compared to tumor growth in sham-hepatectomized controls. The animals were sacrificed every 4 hrs during three days from the 6th to the 78th h following each type of surgery. Unoperated tumor-bearing mice were likewise killed as controls, but only during one complete circadian period. All animals received 2 microg of colchicine per g of body weight intraperitoneally 4 hrs before decapitation. Measurement of mitotic indices in hematoxylineosin-stained tumor samples revealed a decrease in proliferation and a modification of the diurnal mitotic-activity profile in the hepatectomized and sham-operated animals from the first day after surgery. These differences persisted by the third postoperative day only in the hepatectomized animals. Thus, although surgical stress may initially affect tumor growth, the latter results must be the effect of the influence of the hepatic regeneration.