A modification of the in ovo-method for the determination of the antihemorrhagic activity of plant extracts
Glunder, G.; Jantzen, K.; Habermehl, G.G.
Deutsche Tierärztliche Wochenschrift 108(5): 214-215
2001
ISSN/ISBN: 0341-6593 PMID: 11417381 Document Number: 529059
Up to now there has been only a method available for testing the antihaemorrhagic activity of plant extracts, in which chicken embryos had to be transferred to a special container on the fifth day of incubation. Furthermore, the test could not be conducted on the extraembryonal circulatory system after two days. In the test method described here the embryos remain inside the egg. After removal of the egg shell through the air cell and removal of the inner cell membrane, direct access to the extraembryonal circulatory system was made possible, minimizing external influences on the test system. This represents a dramatic simplification in technique and already permits testing on the fourth day of incubation. The application of this method to determine the antihaemorrhagic properties of samples has the advantage of permitting the simple and quick production of haemorrhages by snake venom on the yolk sac circulatory system.