Effect of mutations in PHO85 and PHO4 genes on utilization of proline in Saccharomyces cerevisiae yeasts
Popova, I.G.; Padkina, M.V.; Sambuk, E.V.
Genetika 36(12): 1622-1628
2000
ISSN/ISBN: 0016-6758 PMID: 11190469 Document Number: 525050
Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphorylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and delta-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.