Effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E
Huang, Y.H.; Li, W.M.; Jiang, D.Z.; Wang, Q.R.
Hunan Yi Ke da Xue Xue Bao 25(2): 201-202
2000
ISSN/ISBN: 1000-5625 PMID: 12212225 Document Number: 524694
Serum-free murine bone marrow endothelial cell conditioned medium(mBMEC-cm) was collected. The mBMEC-cm was ultrafiltered in Centriprep. The concentrated retentive substance of mBMEC-cm (MW > 10,000) and filtrate of mBMEC-cm(MW < 10,000) were obtained. The effects of mBMEC-cm on the growth of CFU-E and BFU-E were investigated. The results showed: 1. The retentive substance of mBMEC-cm significantly enhanced the growth of CFU-E and BFU-E. The effects of mBMEC-cm(2%-6%, V/V) on the growth of CFU-E and BFU-E were dose-dependent. 2. The filtrate of mBMEC-cm markedly inhibited the growth of CFU-E and BFU-E. The effects of the filtrate of mBMEC-cm on the growth of CFU-E and BFU-E were also dose-dependent. The mechanism of regulation will be studied further.