Effects of the composition of bone tissue growth factors on cell growth on the model of long term culture of the bone marrow
Volozhin, A.I.; Bondarenko, O.A.; Desiatnichenko, K.S.; Kurdiumov, S.G.; Lebedev, V.G.
Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia 2: 21-23
2003
ISSN/ISBN: 0031-2991 PMID: 12838769 Document Number: 554477
The experimental study of the properties of the collagen- and hydroxyapatite-containing osteoplastic material HAPKOL-I by bone marrow hemopoiesis was made in comparison with existing material HAPKOL. HAPKOL-I is a working name of the material consisting of HAPKOL and composition of non-collagen bone proteins produced from xenogenic bone material. Long-term cultures of bone marrow from mice (C57B1/6xCBA)F1 of dexter type were used. Cultivation time was 7, 14, 21 and 28 days. Intensity of hemopoietic processes was assessed on the samples; absolute count of stromal and hemopoietic cells on the membrane was calculated. It was found that in long-term cultures of the bone marrow stromal and hemopoietic cells develop on the materials HAPKOL and HAPKOL-I, processes of formation of hemopoietic microenvironment provide normal hemopoiesis. On both materials there was a gradual increase of cell counts, a rise in the number of stromal and a fall in the number of hemopoietic cells. More intensive hemopoiesis on the material containing non-collagen bone proteins may be due to this factor.