SV40 can be reproducibly detected in paraffin-embedded mesothelioma samples
Cristaudo, A.; Powers, A.; Vivaldi, A.; Foddis, R.; Guglielmi, G.; Gattini, V.; Buselli, R.; Sensales, G.; Ciancia, E.; Ottenga, F.
Anticancer Research 20(2a): 895-898
2000
ISSN/ISBN: 0250-7005 PMID: 10810373 Document Number: 522439
We studied tissue sections from 18 paraffin embedded mesothelioma specimens diagnosed by the Pathology Department of S. Chiara Hospital of Pisa. Using PCR analysis and Southern blot hybridization we examined the specimens for the DNA regulatory region of the virus. 10/18 (55.5%) of the samples tested contained SV40 DNA regulatory sequences, and of these positive samples, 80% were found to contain Tag sequences by PCR and Southern Blot hybridization. These results confirm that SV40 can be amplified and detected in paraffin embedded mesothelioma samples.