A technical note on microsatellite DNA instability studied in archival paraffin-embedded tissues
Rudzki, Z.; Zazula, M.; Stachura, J.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists 52(4): 225-230
2001
ISSN/ISBN: 1233-9687 PMID: 11915186 Document Number: 534284
The authors present brief overview of problems associated with analysis of microsatellite sequences in DNA from archival paraffin-embedded tissue. This methodology can serve several diagnostic and research purposes provided appropriate precautions are taken to extract DNA of acceptable quality with small admixture of contaminants. Also PCR amplification step requires some modifications compared to routine reactions involving DNA from fresh or freshly-frozen tissues.
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