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Comparative study of soluble and immobilized phenol oxidase from the fungus Mycelia sterilia IBR 35219/2

Mchedlishvili, N.I.; Pruidze, G.N.; Omiadze, N.T.; Zukhbaia, R.V.

Prikladnaia Biokhimiia i Mikrobiologiia 36(2): 138-142

2000

ISSN/ISBN: 0555-1099
PMID: 10779999
Document Number: 521736
Phenol oxidase (EC 1.14.18.1) from the microscopic fungus Mycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH 5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH 5.2). Immobilization considerably increased the enzyme stability. Both soluble and immobilized forms of phenol oxidase from M. sterilia IBR 35219/2 catalyze oxidative conversion of phenolic compounds of the green tea extract.

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