A general method for immobilization of glycoproteins on regenerable immobilized metal-ion carriers: application to glucose oxidase from Penicillium chrysogenum and horseradish peroxidase
Chaga, G.
Biotechnology and Applied Biochemistry 20(1): 43-53
1994
ISSN/ISBN: 0885-4513 PMID: 7917065 Document Number: 434683
A general method for immobilization of glycoproteins on immobilized metal-ion carriers is described. The method includes oxidation of the carbohydrate moiety of the glycoprotein with potassium periodate, covalent modification with histidine and immobilization on bivalent metal ion-iminodiacetic acid-agarose. The method is exemplified with horseradish peroxidase and glucose oxidase from Penicillium chrysogenum. The modified and immobilized enzymes are stable for at least 2 months at 4 degrees C. The immobilized enzymes were used as enzyme electrodes for the determination of the glucose concentration.