Preparation of hepatitis C virus nonstructural region antigens and detection of their relative antibodies
Peng, X.; Wang, Q.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 14(1): 80-84
2000
ISSN/ISBN: 1003-9279 PMID: 11503033 Document Number: 521111
To investigate the relationship between HCV NS3 and the pathogenesis of HCV and research a method to assess the therapeutic effect of interferon (lEN) in clinic. In a retrospective research, we used HCV-NS3 antigens of different terminals expressed in E. coli which contained the recombinant plasmids involving amino terminal region (N') or carboxyl terminal region (C') of HCV-NS3, to detect the antibodies by Western blot in sera of 63 hepatitis C patients who were treated with IFN. It proved that antibodies against HCV-NS3 N' and/or HCV NS3 C' terminals in hepatitis C patients may predict the treatment result of IFN, especially in the patients with chronic hepatitis C. The detection of antibodies against HCV-NS3 N' and/or C' terminals may be used for evaluation of IFN treatment, especially in chronic hepatitis C ones.