Upregulation of adhesion complex proteins and fibronectin by human keratinocytes treated with an aqueous extract from the leaves of Chromolaena odorata (Eupolin)
Phan, T.T.; Allen, J.; Hughes, M.A.; Cherry, G.; Wojnarowska, F.
European Journal of Dermatology Ejd 10(7): 522
2000
ISSN/ISBN: 1167-1122 PMID: 11056422 Document Number: 519341
The effect of Eupolin (Vietnam National Institute of Burns) on the expression of adhesion complex components and fibronectin was investigated. Human keratinocytes were cultured in a serum-free basal medium supplemented with increasing amounts of Eupolin. Indirect immunofluorescence was used to determine whether clinical efficacy might be explained, in part, by an effect of the extract on the adhesion complex proteins of the skin. The results showed that culture in Eupolin-supplemented serum-free basal medium increased the expression of laminin 5, laminin 1, collagen IV and fibronectin. The effect of Eupolin on the expression of these proteins by cultured human keratinocytes was dependent on the dose of Eupolin in the culture medium. The expression of beta 1 and beta 4 integrins was upregulated by the extract at low concentrations (0.1 and 1 micro g/ml), but the expression decreased at higher doses of Eupolin (10 micro g-150 micro g/ml). It is suggested that the increased expression of adhesion complex proteins and fibronectin by Eupolin may contribute to its clinical effectiveness.