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Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis

Osipiuk, J.; Sriram, M.; Mai, X.; Adams, M.W.; Joachimiak, A.

Acta Biochimica Polonica 47(1): 209-214

2000

ISSN/ISBN: 0001-527X
PMID: 10961695
Document Number: 515872
Two genes of the extreme thermophilic archaeon Thermococcus litoralis homologous to those that code for Cpn60 chaperonins were cloned and expressed in Escherichia coli. Each of the Cpn60 subunits as well as the entire Cpn60 complex crystallize in a variety of morphological forms. The best crystals diffract to 3.6 ANG resolution at room temperature and belong to the space group I422 with unit cell parameters a = b = 193.5 ANG, c = 204.2 ANG.

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