Expression of a heterodimeric Fab antibody protein in one cloning step
Mullinax, R.L.; Gross, E.A.; Hay, B.N.; Amberg, J.R.; Kubitz, M.M.; Sorge, J.A.
Biotechniques 12(6): 864-869
1992
ISSN/ISBN: 0736-6205 PMID: 1642894 Document Number: 403190
A new method is presented for creating antibody expression libraries in Escherichia coli. Rather than perform two cloning steps to express the heavy and light chains of the antigen binding domain, we have used a fusion-PCR method to link the coding regions for heavy and light chain sequences in a single DNA molecule prior to vector ligation. This greatly simplifies the construction of antibody expression clones.
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