Application of microsatellite alteration of urine sediment in the early diagnosis of bladder cancer
Qiu, L.; Cong, X.; Tan, Y.
Zhonghua Zhong Liu Za Zhi 22(6): 483-486
2000
ISSN/ISBN: 0253-3766 PMID: 11235570 Document Number: 515747
To assess the usefulness of microsatellite DNA sequence (MS) alterations in urine sediment for early diagnosis of human bladder cancer. Loss of heterozygosity(LOH) and microsatellite instability(MIN) in urine sediment from 28 cases of bladder cancer were detected by polymerase chain reaction (PCR) with selected primers of 10 microsatellite loci. The peripheral blood mononuclear cells and bladder carcinoma cells were used as controls. In 24 of 28 bladder cancer patients (85.7%) LOH and MIN were found in urine sediment on at least one MS locus. Only in 3 of 28 patients(10.7%) was the urine cytology positive while MS and MIN were detected in these 3 patients. The conformance of MS alterations between cancer cells and urine sediment in the same patients was 94.1%. No MS alteration was found in 15 normal controls. Application of microsatellite sequence of urine sediment can be considered as a new tool for screening and early diagnoses of bladder cancer.