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Proteolytic activity of the enzyme complex of the mammalian pancreas in comparison with pancreatin

Berdutina, A.V.; Nekliudov, A.D.; Ivankin, A.I.; Karpo, B.S.; Mitaleva, S.I.

Prikladnaia Biokhimiia i Mikrobiologiia 36(4): 422-427

2000

ISSN/ISBN: 0555-1099
PMID: 10994190
Document Number: 512789
The proteolytic activity and thermal stability of the enzyme complex of cell suspension from pig and bovine pancreas glands was compared with those of pancreatin. The enzyme complex displayed the highest thermal stability and activity at 50 degrees C. The kinetic constants, energies of activation and inactivation of the enzyme complex, and pH optimum (7.0 +/- 0.1) at which this complex had the maximum proteolytic activity were determined. Pancreatin had a pH optimum of 8.0 +/- 0.1.

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