Use of transmembrane current harmonics originating from electrostriction for clarifying mechanisms of ion transport in prepartions of sodium-potassium ATPase
Pasechnik, V.I.
Biofizika 45(6): 1057-1065
2000
ISSN/ISBN: 0006-3029 PMID: 11155233 Document Number: 512542
The mechanisms of generation of transmembrane current harmonics arising due to electrostriction of the bilayer upon applying an alternating electric voltage to sodium/potassium ATPase sorbed on the support was studied. It is discussed what information can be obtained by measurements of transmembrane current harmonics to clarify the mechanism of ion transport in sodium/potassium ATPase preparations. It was shown that for studying the current harmonics upon electrostriction, the preparations should be sorbed on bilayer lipid membranes with a high modulus of elasticity. The kinetics of changes in the amplitude of the second current harmonics in response to an impulse addition of ATP to the preparation can have some specific features depending on the mechanism of ion transport.