Radioimmunoassay for aldosterone and deoxycorticosterone in human plasma. Comparison of various antisera and determination of normal values
Sulon, J.; Palem-Vliers, M.; Genard, P.; Cittanova, N.; Desfosses, B.
Clinica Chimica Acta; International Journal of Clinical Chemistry 72(3): 275-284
1976
ISSN/ISBN: 0009-8981 PMID: 975582 DOI: 10.1016/0009-8981(76)90189-3Document Number: 510984
This paper describes a study of the physico-chemical and radioimmunological properties of three antialdosterone antisera which permitted practical conclusions to be drawn. By its high degree of specificity, anti-aldo-3-oxime-BSA constitutes the most useful antiserum for the clinical assay of aldosterone. The principal advantage of this antiserum is that it allows both urinary and blood aldosterone radioimmunoassay without the necessity of including a chromatographic step. No problems arise with the blanks. This work also includes the study of two anti-deoxycorticosterone antisera. The aldosterone and deoxycorticosterone values, obtained from normal subjects under various physiological conditions, are in agreement with the values given in the literature.