Blood levels of homocysteine by high pressure liquid chromatography and comparison with two other techniques
Ceppa, F.; Drouillard, I.; Chianea, D.; Burnat, P.; Perrier, F.; Vaillant, C.; El Jahiri, Y.
Annales de Biologie Clinique 57(4): 474-480
1999
ISSN/ISBN: 0003-3898 PMID: 10432372 Document Number: 505711
Cardio-vascular diseases are the most common cause of death in industrialized countries. A new marker has emerged among offending risk factors in the past few years: homocysteine. This sulphured amino-acid is an important intermediate in transsulphuration and remethylation reactions of methionine's metabolism. We proposed to evaluate a home made method of determination for this parameter by high performance liquid chromatography (HPLC) and to compare it to fluorescence polarization immunoassay technique (FPIA) and to gaz phase chromatography (CG-SM). This method associated with good sensibility and precision remain much less expensive than FPIA technique.