The effect of a soil extract on the development of Bacillus thuringiensis and on its synthesis of an insecticidal endotoxin
Dregval, O.A.; Cherevach, N.V.; Andrienko, O.E.; Vinnikov, A.I.
Mikrobiolohichnyi Zhurnal 61(4): 40-44
1999
ISSN/ISBN: 0201-8462 PMID: 10565149 Document Number: 503352
Selection of effective and inexpensive nutrient medium for cultivation of entomopathogenic bacteria Bacillus thuringiensis was carried out. The medium with molasses , corn extract and mineral salts has been chosen. Addition of a soil extract to the medium enhanced growth of microorganisms, increased the rate of culture development, the yield of spore-crystalline material and quantity of synthesized endotoxin. Apparently the strengthening effect belongs to humic substances contained in the soil extract.