Differential inhibition by Bacillus thuringiensis delta endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta
Reuveni, M.; Dunn, P.E.
Biochemical and Biophysical Research Communications 181(3): 1089-1093
1991
ISSN/ISBN: 0006-291X PMID: 1662492 Document Number: 368433
Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis .delta. endotoxin for a short period resulted in differential inhibition of K+-dependent transport of leucine relative to the effect on K+-dependent transport of aspartic acid. The difference in I1/2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis .delta. endotoxin to the leucine transport system.