Top Abstract Purchase Related
Home
>
Node
>
Subnode

A test system based on the lux-regulon from Vibrio fischeri for studying the functional activity of mutant forms of Escherichia coli lon-proteinase

Manukhov, I.V.; Eroshnikov, G.E.; Zavil'gel'skiĭ, G.B.; Ginodman, L.M.; Mel'nikov, E.E.; Rotanova, T.V.; Starkova, N.N.; Tsirul'nikov, K.B.

Bioorganicheskaia Khimiia 25(5): 365-368

1999

ISSN/ISBN: 0132-3423
PMID: 10495894
Document Number: 502468
The possibility of application of the bioluminescence method (Lux-test) for studying in vivo functional activity of Escherichia coli protease Lon and its mutants was demonstrated. This assay is based on the capacity of protease Lon and its mutant forms for specific degradation of the LuxR protein, a positive transcriptional activator of the right operon luxICDABE from the marine bacterium Vibrio fischeri, and thus to affect the level of AB luciferase in the cells. A correlation between in vitro activity of the protease Lon mutants and the intensity of bioluminescence measured by the Lux-test was revealed.

Document emailed within 1 workday
Secure & encrypted payments

Zavil'gel'skiĭ, G.B.; Eroshnikov, G.E.; Manukhov, I.V.; Rasulova, F.S.; Ginodman, L.M.; Mel'nikov, E.E.; Starkova, N.N.; Rotanova, T.V. 1999: In vivo analysis of the proteolytic activity and effects of "sequestering" and negative domination of Escherichia coli lon-mutants using the lux regulon of Vibrio fischeri Molekuliarnaia Biologiia 33(5): 797-802
Gholizadeh, A. 2016: Differential expression of a cysteine proteinase and cystatin pair as side­by­side fusion forms in Escherichia coli Tsitologiia i Genetika 50(4): 16-25
Chebotarev, D.V.; Gul'ko, L.B.; Veĭko, V.P. 2001: Protein engineering of uridine phosphorylase from Escherichia coli K-12. II. Comparative study of hybrid and mutant forms of uridine phosphorylases Bioorganicheskaia Khimiia 27(3): 184-190
Marino, A.; Crisafi, G.; Maugeri, T.L.; Nostro, A.; Alonzo, V. 1999: Uptake and retention of Vibrio cholerae non-O1, Salmonella typhi, Escherichia coli and Vibrio harvey by mussels in seawater New Microbiologica 22(2): 129-138
Nagoev, B.S.; Shubich, M.G. 1981: Value of the nitroblue tetrazolium reduction test in studying the functional activity of leukocytes (review of the literature) Laboratornoe Delo 1981(4): 195-198
Aĭrapetian, S.N.; Stepanian, R.S.; Oganesian, G.G.; Barsegian, A.A.; Alaverdian, Z.R.; Arakelian, A.G.; Markosian, L.S. 2001: Effect of mechanical stress on lon mutant strain of Escherichia coli K-12 Mikrobiologiia 70(2): 248-252
Morris, D.D.; Cullor, J.S.; Whitlock, R.H.; Wickstrom, M.; Corbeil, L.B. 1986: Endotoxemia in neonatal calves given antiserum to a mutant Escherichia coli (J-5) American Journal of Veterinary Research 47(12): 2554-2565
Ghose, A.C. 1996: Adherence and colonization properties of Vibrio cholerae and diarrhoeagenic Escherichia coli Indian Journal of Medical Research 104: 38-51
Ruíz-Herrera, J.; Ramírez, A.; Villarreal-Moguel, E.I. 1979: Synthesis of the nitrate reductase complex in a constitutive hem mutant of Escherichia coli K-12 Revista Latinoamericana de Microbiologia 21(4): 193-197
Tamari, M.; Horiguchi, M. 1976: Degradation of ciliatine (2-aminoethylphosphonic acid) by the mutant strain Tm-1 of Escherichia coli Seikagaku. Journal of Japanese Biochemical Society 48(8): 810-813
Brouillard, M.Y.; Rateau, J.G. 1990: Cholestyramine adsorbs Escherichia coli and Vibrio cholerae toxins by way of ion exchange mechanism Annales de Gastroenterologie et d'Hepatologie 26(1): 27-30
Brouillard, M.Y.; Rateau, J.G. 1990: Cholestyramine adsorbs Escherichia coli and Vibrio cholerae toxins by way of ion exchange mechanism Annales de Gastroenterologie et d'Hepatologie 26(1): 27-30
Ben OmraneE, I.B.; Trabelsi, S.; Mraouna, R.; El Bour, M. 2013: Comparative study of survival Vibrio parahaemolyticus with Escherichia coli and Salmonella typhimurium in seawater Archives de l'Institut Pasteur de Tunis 90(1-4): 47-54
Verbenko, V.N.; Kuznetsova, L.V.; Kalinin, V.L. 1994: Mutant alleles for radioresistance form the Escherichia coli strain Gam(r)444): cloning and preliminary characteristics Genetika 30(6): 756-762
Ptitsyn, L.R.; Al'tman, I.B.; Gurov, M.V. 1998: Expression of synthetic human interleukin-10 gene and its mutant variants in Escherichia coli cells Bioorganicheskaia Khimiia 24(1): 48-57
Androsov, V.V.; Demkin, V.V.; Levashev, V.S. 1977: Morphologic mutant of Escherichia coli K12 having disorders in morphology, division and cell wall biosynthesis Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1977(4): 53-59
Fumarola, D. 1977: Preliminary observations on the possible use of platelets as a cellular system in order to check the enteropathogenic activity of Escherichia coli and other enterobacteria (author's transl Pathologica 69(987-988): 65-75
Miriuta, A.I.; Pererva, T.P. 2008: Biological activity of Ungernia victoris extract in the Escherichia coli CaCl2-transformation system in the presence of calcium channel modulators Tsitologiia i Genetika 42(4): 45-49
Belitskiĭ, B.R.; Shakulov, R.S. 1988: Cloning of the gpp gene of Escherichia coli and the use of recBC, sbcB cells for inserting its mutant allele into the chromosomal structure Genetika 24(8): 1333-1342
Baranova, N.A.; Egorov, N.S. 1989: Production of antibiotic substances by natural variants of the marine bacterium Vibrio Fischeri Antibiotiki i Khimioterapiia 34(1): 7-10