Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma
Ragazzini, P.; Gamberi, G.; Benassi, M.S.; Orlando, C.; Sestini, R.; Ferrari, C.; Molendini, L.; Sollazzo, M.R.; Merli, M.; Magagnoli, G.; Bertoni, F.; Bohling, T.; Pazzagli, M.; Picci, P.
Cancer Detection and Prevention 23(2): 129-136
1999
ISSN/ISBN: 0361-090X PMID: 10101594 Document Number: 499303
The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.