Orthopoxvirus genes for Kelch-like proteins. I. Analysis of species specific differences by gene structure and organization
Totmenin, A.V.; Kolosova, I.V.; Shchelkunov, S.N.
Molekuliarnaia Biologiia 36(4): 610-616
2002
ISSN/ISBN: 0026-8984 PMID: 12173463 Document Number: 540581
Genes and proteins of the kelch superfamily were structurally analyzed in the smallpox (SPV), monkeypox (MPV), cowpox (CPV), and vaccinia (VV) viruses. Genes potentially coding for the kelch-like proteins were found only in the variable terminal regions of the orthopoxvirus genome. The set and sizes of their protein products varied with species. All genes of the superfamily proved to be disrupted by mutations in SPV, which is highly pathogenic for its only host, man. The largest set of kelch-like proteins was observed for CPV, which is low-pathogenic for humans and has the broadest animal host range. The kelch-like proteins of one virus showed low homology to each other, whereas isologs of different viruses were highly homologous. The results testified to the earlier assumption that CPV is the most ancient and an ancestor of the other orthopoxviruses pathogenic for humans.