The Bop gene adjacent to the mouse CD8b gene encodes distinct zinc-finger proteins expressed in CTLs and in muscle
Hwang, I.; Gottlieb, P.D.
Journal of Immunology 158(3): 1165-1174
1997
ISSN/ISBN: 0022-1767 PMID: 9013956 Document Number: 476555
The Bop gene (CD8b opposite) is located immediately upstream of the mouse CD8b gene. Expression of Bop gene transcripts has been observed in several long-term cytotoxic T lymphocyte (CTL) lines and in the thymus. In the present work, expression of the Bop gene in lymphocytes appeared to be confined to CD8-positive cells, and expression of Bop was inducible by concanavalin A. It was shown that single Bop gene encoded protein products with distinct amino-terminal sequences that were expressed in CTLs (t-BOP) and in cardiac and skeletal muscle (skm-BOP), as well as what appeared to be a non-coding cDNA (t-ncb) expressed only in CTLs. The t-BOP and t-ncb cDNAs in CTLs resulted from alternative splicing of a single primary transcript, whereas the Bop transcripts expressed in CTLs and in muscle appeared to be transcribed from different promoters. The BOP proteins expressed in CTLs and muscle contained zinc finger-like motifs with homology to those of the ETO/MTG8 proto-oncogene and several other proteins of interest. Western blot analysis with a hamster anti-BOP monoclonal antibody detected the BOP protein in muscle cells and in COS 7 cells transfected with Bop cDNA constructs. DNA sequences of t-Bop, t-ncb, skm-Bop1 and skm-Bop2 have been submitted to GenBank under accession numbers U76371, U76372, U76373 and U76374 respectively.