New DNA diagnostic system for determination and identification of homo- and heterozygote point mutations
Patrushev, L.I.; Zykova, E.S.; Kaiushin, A.L.; Korosteleva, M.D.; Miroshnikov, A.I.
Bioorganicheskaia Khimiia 24(3): 194-200
1998
ISSN/ISBN: 0132-3423 PMID: 9612561 Document Number: 496858
A straightforward and effective PCR-based assay system is devised that allows one to reveal and identify homozygous and heterozygous point mutations. The system uses two sets of allele-specific primers. In one set, the 3'-nucleotide matches the allele under study so that the primer functions effectively only if the DNA contains the corresponding allele. To increase primer specificity, template-noncomplementary nucleotides are introduced near its 3'-end. The primers from another set invariably bear a 3'-terminal mismatch, and, in addition, the mutant nucleotides of the alleles under study form mismatches with the internal nucleotides of the primers. In such combination, the primer activity is suppressed if the DNA contains a homozygous mutation. The assay system devised was utilized to reveal the Leiden mutation in the gene for factor V of the human blood clotting system in patients with thrombophilia.