Analysis of point mutations of Ha-ras gene in basaliomas and spinaliomas usiang the polymerase chain reaction (PCR) technic
Peris, K.; Cerroni, L.; Chimenti, S.; Soyer, H.P.; Kerl, H.; Höfler, H.
Giornale Italiano di Dermatologia e Venereologia Organo Ufficiale Societa Italiana di Dermatologia e Sifilografia 124(10): 429-434
1989
ISSN/ISBN: 0392-0488 PMID: 2635687 Document Number: 331620
Polymerase chain reaction is a recently developed procedure to detect point mutations in human genomic DNA. The sensitivity and specificity of this technique which requires a very little amount of DNA, have allowed the large scale screening of tumors. The Authors report the detection of point mutations of Ha-ras gene at codon 12 in 12 basal cell carcinomas and 10 cutaneous squamous cell carcinomas by the use of polymerase chain reaction in paraffin-embedded tissue sections. The gene alteration was identified in 2 basaliomas whereas no squamous cell carcinoma showed point mutations. These results suggest that point mutations of Ha-ras gene do not represent a general activating mechanism in cutaneous epitheliomas.